Journal:

Article Title: Galactose and Lactose Genes from the Galactose-Positive Bacterium Streptococcus salivarius and the Phylogenetically Related Galactose-Negative Bacterium Streptococcus thermophilus : Organization, Sequence, Transcription, and Activity of the gal Gene Products

doi: 10.1128/JB.184.3.785-793.2002

Figure Lengend Snippet: Strains and plasmids

Article Snippet: For the determination of sugar concentrations in the media of growing cells, samples (0.25 to 0.5 ml) were taken at intervals, heated at 100°C for 10 min, and centrifuged to remove cells and the supernatants were stored at −20°C until used for sugar assays. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant genotype or characteristics Source or reference Strains E. coli TOP10 F − mcrA Δ( mrr-hsdRMS-mcrBC ) φ80 lacZ ΔM15 Δ lacX74 deoR recA1 araD139 Δ( ara-leu7697 ) galU rpsL endAl nupG Invitrogen S. salivarius ATCC 25975 Wild type, Lac + Glu + Gal + 12 S. thermophilus SMQ-301 Wild type, Lac + Glu − Gal − 32 Plasmids pUC18 Cloning vector, Ap r lacZ ′ ( lacZ α) 37 pCR-Blunt Cloning vector Invitrogen PCR 2.1 TOPO Cloning vector, Ap r Ka r lacZ α Invitrogen pGAL1 Contains the last 1,040 nucleotides of galT and the first 526 nucleotides of galE from S. salivarius cloned into pCR-Blunt This work pGAL3 Contains the last 650 nucleotides of galK and the first 1,202 nucleotides of galT from S. salivarius cloned into pCR-Blunt This work pGAL29 Contains the first 882 nucleotides of galK and the first 853 nucleotides of galR from S. salivarius cloned into PCR 2.1 TOPO This work pGAL73 Contains the last 1,422 nucleotides of dexB , galR , and the first 729 nucleotides of galK from S. salivarius cloned into pUC18 This work pGAL123 Contains the last 104 nucleotides of galT , galE , and the first 928 nucleotides of galM from S. salivarius cloned into pUC18 This work Open in a separate window Strains and plasmids DNA purification and manipulations.

Techniques: Plasmid Preparation, Clone Assay

Journal:

Article Title: Identification of Genes Regulated by the cepIR Quorum-Sensing System in Burkholderia cenocepacia by High-Throughput Screening of a Random Promoter Library ▿

doi: 10.1128/JB.01201-06

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Antibiotics were purchased from Sigma-Aldrich Canada Ltd. (Oakville, ON, Canada). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Description a Source or reference E. coli strains DH5α F − φ80 lacZ Δ M15 Δ( lac ZYA-argF ) U169 rec A1 endA gyrA96 thi-1 hsdR17 supE44 relA1 deo R Invitrogen TOP10 F − mcrA Δ( mrr-hsd RMS-mcr BC ) φ80 lacZ Δ M15 Δ lacX74 deo R rec A1 ara D139 Δ ( ara-leu ) 7697 gal U galK rpsL (Str r ) endA1 nupG Invitrogen B. cenocepacia strains K56-2 Cystic fibrosis respiratory isolate 28 K56-R2 cepR ::Tn 5 -OT182 derivative of K56-2, Tc r 23 K56-dI2 Δ cepI derivative of K56-2 31 Plasmids pCR2.1TOPO Cloning vector for PCR products, Ap r Km r Invitrogen pBS7 pCR2.1TOPO containing 1.3-kb ndh fragment 14 pMS402 lux - based promoter reporter plasmid, Km r Tp r 12 pCC1 pMS402 containing a zmpA :: luxCDABE fusion, Km r Tp r 40 pSLS225 pUCP26 with 1.5-kb SphI-KpnI fragment containing cepI , Tc r 25 pRM445 pMS402 containing a cepR :: luxCDABE transcriptional fusion, Km r Tp r 31 pCP300 pMS402 containing a cepI :: luxCDABE transcriptional fusion, Km r Tp r 31 pMVbcscV pMS402 containing a bcscV :: luxCDABE transcriptional fusion, Km r Tp r This study pBS18 pMS402 containing a katB::luxCDBAE transcriptional fusion, Km r Tp r pVDA01 pMS402 containing a pvdA :: luxCDBAE transcriptional fusion, Km r Tp r This study pVDO301 pMS402 containing an orbI :: luxCDBAE transcriptional fusion, Km r Tp r This study Open in a separate window a Ap, ampicillin; Km, kanamycin; Tp, trimethoprim; Tc, tetracycline.

Techniques: Plasmid Preparation, Clone Assay